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Boster Bio
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NeuroMab
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Image Search Results
Journal: Annals of clinical and translational neurology
Article Title: A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient.
doi: 10.1002/acn3.52262
Figure Lengend Snippet: Figure 3. Analysis of GABAAR pentameric structure. (A) A pentameric model featuring 2 a2 subunits (GABRA2), 2 b3 subunits (GABRB3), and 1 c2 subunit (GABRG2). (B) The structure is rotated 90 degrees for a top-down view. In the wild-type GABRA2, Ala308 forms hydrogen bonds with Pro209 of GABRB3 and with another GABRA2 protein at position 238 of GABRG2. (C) In the mutated GABRA2 (p.Ala308Val), the longer valine side chain disrupts these hydrogen bonds, potentially altering structural stability.
Article Snippet: The primary antibodies utilized included: DYKDDDDK Tag (9A3) Mouse mAb (Cat#8146, Cell Signaling Technology, MA, USA, diluted 1:200); GABRB3 Antibody (Cat#PB0627, Boster Biological Technology, Wuhan, China, diluted 1:1000);
Techniques:
Journal: Annals of clinical and translational neurology
Article Title: A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient.
doi: 10.1002/acn3.52262
Figure Lengend Snippet: Figure 5. In vitro functional assay. (A) Western blotting analysis indicated that the GABRA2-MUT group exhibited protein expression, but it was significantly lower than the GABRA2-WT group, with a reduction of approximately 70.62% (***P < 0.001). (B) CO-IP results showed that wild-type GABRA2 interacted with GABRB3/GABRG2, while the mutant may disrupt this interaction. The right panel presents normalized CO-IP analysis, ***P < 0.001.
Article Snippet: The primary antibodies utilized included: DYKDDDDK Tag (9A3) Mouse mAb (Cat#8146, Cell Signaling Technology, MA, USA, diluted 1:200); GABRB3 Antibody (Cat#PB0627, Boster Biological Technology, Wuhan, China, diluted 1:1000);
Techniques: In Vitro, Functional Assay, Western Blot, Expressing, Co-Immunoprecipitation Assay, Mutagenesis